IL-1RN and IL-1βPolymorphism and ARV-Associated Hepatotoxicity

The severity of hepatic injury depends upon cytokines. Previous studies associatedIL-1RNallele 2 withIL-1βproduction. Hence, we examined the association ofIL-1 RNandIL-1βpolymorphisms with ARV-associated hepatotoxicity. Genotyping ofIL-1RN(VNTR),IL-1β(-511C/T) polymorphisms was done in 162 HIV-infected patients, 34 with ARV hepatotoxicity, 128 without hepatotoxicity, and 152 healthy controls using PCR and PCR-RFLP method. The haplotypes 1T and 2C enhanced the risk for severe hepatotoxicity (OR=1.41,P=0.25;OR=1.67,P=0.31).IL-1β-511TT genotype significantly represented among tobacco using HIV-infected individuals compared to nonusers (OR=3.74,P=0.05).IL-1β-511TT genotype among alcohol users increased the risk for hepatotoxicity (OR=1.80,P=0.90).IL-1β-511CT and-511TT genotypes overrepresented in alcohol using HIV-infected individuals (OR=2.29,P=0.27;OR=2.64,P=0.19).IL-RN2/2 and 1/3 genotypes represented higher in nevirapine using hepatotoxicity patients (OR=1.42,P=0.64,OR=8.79,P=0.09).IL-1β-511CT and-511 TT genotypes among nevirapine users enhanced the risk for severe hepatotoxicity (OR=4.29,P=0.20;OR=1.95,P=0.56).IL-1β-511CT and-511TT genotypes were overrepresented in combined nevirapine and alcohol using HIV-infected individuals as compared to nevirapine users and alcohol nonusers (OR=2.56,P=0.26;OR=2.84,P=0.24).IL-1β-511TT genotype with tobacco, alcohol, and nevirapine usage revealed a trend of risk for the development of ARV-associated hepatotoxicity and its severity.