GTG banding pattern on human metaphase chromosomes revealed by high resolution atomic‐force microscopy
- 1 June 2001
- journal article
- Published by Wiley in Journal of Microscopy
- Vol. 202 (3) , 464-467
Abstract
Surface topography of human metaphase chromosomes following GTG banding was examined using high resolution atomic force microscopy (AFM). Although using a completely different imaging mechanism, which is based on the mechanical interaction of a probe tip with the chromosome, the observed banding pattern is comparable to results from light microscopy and a karyotype of the AFM imaged metaphase spread can be generated. The AFM imaging process was performed on a normal 2n = 46, XX karyotype and on a 2n = 46, XY, t(2;15)(q23;q15) karyotype as an example of a translocation of chromosomal bands.Keywords
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