Mist trickling bioreactor for Centaurium erythraea Rafn growth of shoots and production of secoiridoids
- 1 May 2005
- journal article
- Published by Springer Nature in Biotechnology Letters
- Vol. 27 (10) , 721-724
Abstract
Shoots of Centaurium erythraea Rafn were cultivated in 5 l mist trickling bioreactor for 21 and 28 days increasing their dry weight from 0.54 g to 13.7 g and 18.3 g, respectively. About 6880 shoots from 223 initial shoot-tips in 21-day bioreactor producing cycle were produced. The shoots could be successfully rooted and transferred to soil. Secoiridoid accumulation (expressed as a sum of gentiopicroside, sweroside and swertiamarin) in shoots after 21 days of culture reached about 303 mg l−1.Keywords
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