Large-scale exploration of growth inhibition caused by overexpression of genomic fragments in Saccharomyces cerevisiae
Authors:
- Jeanne BoyerJeanne Boyer
- Gwenaël BadisGwenaël Badis
- Cécile FairheadCécile Fairhead
- Emmanuel TallaEmmanuel Talla
- Florence HantrayeFlorence Hantraye
- Emmanuelle FabreEmmanuelle Fabre
- Gilles FischerGilles Fischer
- Christophe HennequinChristophe Hennequin
- Romain KoszulRomain Koszul
- Ingrid LafontaineIngrid Lafontaine
- Odile Ozier-KalogeropoulosOdile Ozier-Kalogeropoulos
- Miria RicchettiMiria Ricchetti
Open Access
- 31 August 2004
- journal article
- research article
- Published by Springer Nature in Genome Biology
- Vol. 5 (9) , R72
Abstract
We have screened the genome of Saccharomyces cerevisiae for fragments that confer a growth-retardation phenotype when overexpressed in a multicopy plasmid with a tetracycline-regulatable (Tet-off) promoter. We selected 714 such fragments with a mean size of 700 base-pairs out of around 84,000 clones tested. These include 493 in-frame open reading frame fragments corresponding to 454 distinct genes (of which 91 are of unknown function), and 162 out-of-frame, antisense and intergenic genomic fragments, representing the largest collection of toxic inserts published so far in yeast.Keywords
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