Fluorescence polarization: measurements with a photon-counting photometer
Authors:
- 1 April 1978
- journal article
- conference paper
- Published by AIP Publishing in Review of Scientific Instruments
- Vol. 49 (4) , 510-514
Abstract
A T‐format, photon‐counting polarization photometer was constructed to facilitate precise polarization measurements at low signal‐to‐noise ratios. The instrument optics and electronics are described. Several examples of the instrument’s performance are given, including measurements on picomolar fluorescein solutions at low excitation resolution and the excitation polarization spectrum of indole (2×10−4M) at a resolution of 5 Å.Keywords
This publication has 20 references indexed in Scilit:
- RESOLUTION OF THE FLUORESCENCE EXCITATION SPECTRUM OF INDOLE INTO THE 1La AND 1Lb EXCITATION BANDS*Photochemistry and Photobiology, 1977
- Fluorescence polarization microscope for automatic screening of membrane fluidity of individual cellsApplied Optics, 1977
- Digital, photon-counting fluorescence polarometerAnalytical Chemistry, 1976
- Interactions of fluorescent analogs of adenine nucleotides with coupling factor protein isolated from spinach chloroplastsFEBS Letters, 1975
- Description of an apparatus for the simultaneous measurement of changes in the components of polarized fluorescence emitted by membranesJournal of Physics E: Scientific Instruments, 1975
- A SPECTROPHOTOFLUOROMETER FOR MEASURING VERY WEAK FLUORESCENCES FROM BIOLOGICAL MOLECULESPhotochemistry and Photobiology, 1974
- Recording polarization of fluorescence spectrometer. Unique application of piezoelectric birefringence modulationAnalytical Chemistry, 1974
- Versatile Spectrophotofluorometer-Polarization FluorometerReview of Scientific Instruments, 1968
- An Automatic Recording Polarization SpectrofluorimeterApplied Optics, 1964
- Photoelectric Method for the Measurement of the Polarization of the Fluorescence of SolutionsJournal of the Optical Society of America, 1956