In vitro release of interferon‐γ by trichophytin‐stimulated whole blood cell cultures from ringworm‐vaccinated and control calves experimentally inoculated with Trichophyton verrucosum

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Abstract
Of a group of seven calves, four were vaccinated against bovine ringworm with the vaccine Ringvac bovis LTF‐130 (Alpharma, Oslo, Norway), while three calves were left unvaccinated. All calves were inoculated epicutaneously with a virulent strain of Trichophyton verrucosum. Clinical signs were recorded. In response to stimulation with trichophytin, in vitro interferon‐γ (IFN‐γ) production in whole blood cell cultures was assessed in samples obtained pre‐and post‐vaccination and pre‐ and post‐inoculation. A commercial enzyme immunoas‐say kit was used to measure IFN‐γ levels (Bovigam, CSL, Victoria, Australia). Control calves developed typical ringworm lesions, whereas vaccinated calves did not. Following vaccination, release of IFN‐γ in whole blood cell cultures indicated the presence of circulating trichophytin‐specific lymphocytes. After inoculation with T. verrucosum, IFN‐γ production was demonstrated in samples from both vaccinated and control calves. This study showed that vaccination with Ringvac bovis LTF‐130 elicits a protective immune response suggesting involve‐ment of the cellular branch of the immune system. Experimental infection of naïve nonvaccinated calves with T. verrucosum, also indicated stimulation of a cell‐mediated immune response essential for resolution of lesions.

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