Cellulolytic and hemicellulolytic bacteria from the gut of Oryctes rhinoceros larvae

Authors:
  • SSSiti Lusi Arum Sari
    Siti Lusi Arum Sari
  • APArtini Pangastuti
    Artini Pangastuti
  • TPTjahjadi Purwoko
    Tjahjadi Purwoko
  • EMEdwi Mahajoeno
    Edwi Mahajoeno
  • WHWahyu Hidayat
    Wahyu Hidayat
  • IMIkow Mardhena
    Ikow Mardhena
  • DKDewi Kurniawati
    Dewi Kurniawati
  • RARobiah Anitasari
    Robiah Anitasari
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Abstract
Sari SLA, Pangastuti A, Susilowati A, Purwoko Tj, Mahajoeno E, Hidayat W, Mardhena I, Panuntun DF, Kurniawati D, Anitasari R. 2016. Cellulolytic and hemicellulolytic bacteria from the gut of Oryctes rhinoceros larvae. Biodiversitas 17: 78-83. Lignocellulose is very potential as raw material for biofuel production because it is cheap, abundant and renewable. The main carbohydrate constituents of lignocellulosic material are cellulose and hemicelluloses rd instars were used in this research. The research succeeded to isolate 11 bacterial isolates from the gut of O. rhinoceros larvae. The screening result demonstrated that bacterial isolates had cellulolytic (72.7% of total isolates), and mannanolytic (100% of total isolates) activity. Based on the 16S rDNA sequence, 10 isolates were classified into Bacillus and only 1 isolate was classified into Citrobacter. The GOR2 which was closely related to Bacillus pumilus vit bac1 has the highest cellulolytic and xylanolytic activities. The isolate with the highest mannanolytic activity was the GOR7 which was closely related to Bacillus aryabhattai strain IHB B 6821.