Gypsy-like retrotransposons in Pyrenophora: an abundant and informative class of molecular markers
Authors:
- Emily J.A TaylorETEmily J.A Taylor
- Pavlina KonstantinovaPKPavlina Konstantinova
- Fiona LeighFLFiona Leigh
- Jayne A BatesJBJayne A Bates
- David LeeDLDavid Lee
- 1 June 2004
- journal article
- Published by Canadian Science Publishing in Genome
- Vol. 47 (3) , 519-525
Abstract
This paper describes the development of S-SAP (sequence-specific amplified polymorphism) using a primer derived from the LTR (long terminal repeat) of the Pyggy retrotransposon isolated from Pyrenophora graminea. Fragments were amplified by S-SAP from different Pyrenophora spp., indicating the presence of Pyggy-like sequences in these genomes. The bands were highly polymorphic between isolates and the number of bands differed by as much as 10-fold between species, demonstrating the potential of this method for genetic analysis in fungi. The phylogenetic relationship among the isolates as deduced using S-SAP data is presented, and shows evidence of genetic exchange between P. graminea and P. teres.Key words: Pyrenophora, retrotransposon, molecular markers, S-SAP.Keywords
This publication has 13 references indexed in Scilit:
- Rapid‐cycle PCR detection of Pyrenophora graminea from barley seedPlant Pathology, 2001
- Pea Ty1-copia group retrotransposons: transpositional activity and use as markers to study genetic diversity in PisumMolecular Genetics and Genomics, 2000
- A Contiguous 66-kb Barley DNA Sequence Provides Evidence for Reversible Genome ExpansionGenome Research, 2000
- REAL, an LTR retrotransposon from the plant pathogenic fungus Alternaria alternataMolecular Genetics and Genomics, 2000
- Gypsy‐like retrotransposons are widespread in the plant kingdomThe Plant Journal, 1998
- Genetic distribution of Bare–1-like retrotransposable elements in the barley genome revealed by sequence-specific amplification polymorphisms (S-SAP)Molecular Genetics and Genomics, 1997
- AFLP: a new technique for DNA fingerprintingNucleic Acids Research, 1995
- A copia-like element in Pisum demonstrates the uses of dispersed repeated sequences in genetic analysisPlant Molecular Biology, 1990
- Ty elements transpose through an RNA intermediateCell, 1985
- Evidence for transposition of dispersed repetitive DNA families in yeastCell, 1979