In vivo assembly of regularly spaced nucleosomes on mouse βmaj-globin DNA cloned in an SV40 recombinant
- 1 December 1982
- journal article
- research article
- Published by Portland Press Ltd. in Bioscience Reports
- Vol. 2 (12) , 1031-1040
Abstract
Short-term transformation of HeLa cells with an SV40 recombinant carrying a 7.0-Kb [kilobase] mouse genomic globin DNA was studied. By 48 h after transfection, the donor DNA was present in the cell nucleus at high copy episomal numbers with the globin gene in regularly spaced nucleosomal form and transcribed into 9S poly A+ RNA. These minichromosomes can be isolated in sufficient quantity to allow further biochemical and EM studies.Keywords
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