Identification of an S-RNase binding protein1 (SBP1) homolog of apple (Malus^|^times;domestica)
- 1 January 2013
- journal article
- research article
- Published by Japanese Society for Plant Cell and Molecular Biology in Plant Biotechnology
- Vol. 30 (2) , 119-123
Abstract
Many flowering plants exhibit self-incompatibility (SI) to prevent inbreeding and promote outcrossing. This self/non-self discrimination mechanism is controlled by the S locus, which contains separate genes for pistil and pollen specificities. In the gametophytic SI (GSI) of Rosaceae, Solanaceae and Plantaginaceae, the pistil S determinant, S-RNase, encodes extracellular ribonuclease which is thought to act as a cytotoxin to the self pollen tube, while the pollen S determinant is the F-box gene called SLF/SFB/SFBB. In Petunia (Solanaceae), SLF is reported to be a component of the noncanonical E3 ubiquitin ligase complex with S-RNase binding protein1 (SBP1) and Cullin1 (CUL1), and interact with non-self S-RNases to ubiquitinate them for degradation. Here, we isolated an apple (Malus×domestica) homolog of SBP1 (MdSBP1) from pollen RNA by RT-PCR. MdSBP1 included a RING-HC domain required for E3 ubiquitin ligase activity, and showed 64.0–68.2% amino acid identities with solanaceous SBP1 proteins. Expression analysis showed that MdSBP1 was expressed in all the organs analyzed. We detected an interaction between recombinant MdSBP1 protein and S-RNase of apple using a pull-down assay.Keywords
This publication has 35 references indexed in Scilit:
- MEGA5: Molecular Evolutionary Genetics Analysis Using Maximum Likelihood, Evolutionary Distance, and Maximum Parsimony MethodsMolecular Biology and Evolution, 2011
- S-RNase-based self-incompatibility in Petunia inflataAnnals of Botany, 2010
- Pollen-expressed F-box gene family and mechanism of S-RNase-based gametophytic self-incompatibility (GSI) in RosaceaeSexual Plant Reproduction, 2009
- S Locus F-Box Brothers: Multiple and Pollen-Specific F-Box Genes WithSHaplotype-Specific Polymorphisms in Apple and Japanese PearGenetics, 2007
- Identification and Characterization of Components of a PutativePetunia S-Locus F-Box–Containing E3 Ligase Complex Involved in S-RNase–Based Self-IncompatibilityThe Plant Cell, 2006
- The F-Box Protein AhSLF-S2 Controls the Pollen Function of S-RNase–Based Self-IncompatibilityThe Plant Cell, 2004
- Identification of the pollen determinant of S-RNase-mediated self-incompatibilityNature, 2004
- Style‐specific self‐compatible mutation caused by deletion of the S‐RNase gene in Japanese pear (Pyrus serotina)The Plant Journal, 1997
- CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choiceNucleic Acids Research, 1994
- Self-incompatibility in Nicotiana alata involves degradation of pollen rRNANature, 1990