Production of Recombinant Adeno‐Associated Viral Vectors for In Vitro and In Vivo Use
- 15 April 2007
- journal article
- review article
- Published by Wiley in Current Protocols in Molecular Biology
- Vol. 78 (1) , 16.25.1-16.25.24
Abstract
Adeno‐associated virus is a nonpathogenic human virus that has been developed into a gene‐delivery vector due to its high efficiency of infection in many different cell types and its ability to persist and lead to long‐term gene expression. The vector is also a valuable tool in molecular biology experiments. This unit describes efficient methods to generate high‐titer, research‐grade, adenovirus‐free, recombinant single‐stranded and self‐complementary adeno‐associated virus in various serotypes, along with methods to quantify the viral vectors. Keywords: adeno‐associated virus; gene delivery; purification; serotypes; transfection; viral vectorsKeywords
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