Activity Maintenance Characteristics and Protease Adsorption on Langmuir Monolayer of Organo‐Modified Single‐Walled Carbon Nanotubes

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Abstract
The activity maintenance characteristics of proteases adsorbed and immobilized on a monolayer of organo‐modified single‐walled carbon nanotubes (SWCNTs) were evaluated in this study. After forming a monolayer on the water surface of the organo‐modified SWCNTs, protease was introduced into the subphase, and monolayer immobilization was attained by post‐adsorption. The protease‐adsorbed organo‐SWCNT multilayers were immersed in an aqueous solution of luminescent casein, and fluorescence emission was observed. Proteases had cleaved the molecular chain of luminescent casein, which causes luminescence. Although the protease in the aqueous solution could cleave the molecular chain only up to approximately room temperature, the protease immobilized on the solid substrate could cleave the chain up to 160 °C, indicating sharp emission spectra.
Funding Information
  • Ministry of Education, Culture, Sports, Science and Technology (21K05180)

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