A TATA box implicated in E1A transcriptional activation of a simple adenovirus 2 promoter
Authors:
- Lily WuLWLily Wu
- Debra S. E. RosserDRDebra S. E. Rosser
- Martin C. SchmidtMSMartin C. Schmidt
- Arnold BerkABArnold Berk
- 1 April 1987
- journal article
- Published by Springer Nature in Nature
- Vol. 326 (6112) , 512-515
Abstract
Adenovirus E1A proteins stimulate transcription by RNA polymerases II and III from many promoters. The detailed mechanism of transcriptional activation (transactivation) by E1A proteins remains unclear, but genetic and biochemical results suggest that E1A products might act to stimulate the activity of cellular transcription factors. In this study, a detailed mutational analysis of the adenovirus E1B promoter was undertaken to define the DNA sequences required for proper basal transcription and E1A transactivation. Two key findings emerged: first the E1B promoter is an unusually simple RNA polymerase II promoter requiring only two sequence elements for proper regulation, the TATA box and a binding site for transcription factor Sp1; and second only mutations in the TATA box interfere with E1A-transactivation, suggesting that E1A mediates its effect on this promoter through the TATA-box transcription factor.Keywords
This publication has 33 references indexed in Scilit:
- The adenovirus type 5 E1A enhancer contains two functionally distinct domains: One is specific for E1A and the other modulates all early units in cisCell, 1986
- Interaction of a gene-specific transcription factor with the adenovirus major late promoter upstream of the TATA box regionCell, 1985
- Complete transformation by adenovirus 2 requires both E1A proteinsCell, 1984
- Inhibition of RNA cleavage but not polyadenylation by a point mutation in mRNA 3′ consensus sequence AAUAAANature, 1983
- The adenovirus type 5 E1A transcriptional control region contains a duplicated enhancer elementCell, 1983
- Organization and Expression of Eucaryotic Split Genes Coding for ProteinsAnnual Review of Biochemistry, 1981
- Pre-early adenovirus 5 gene product regulates synthesis of early viral messenger RNAsCell, 1979
- Sizing and mapping of early adenovirus mRNAs by gel electrophoresis of S1 endonuclease-digested hybridsCell, 1977
- Characteristics of a Human Cell Line Transformed by DNA from Human Adenovirus Type 5Journal of General Virology, 1977
- Selective extraction of polyoma DNA from infected mouse cell culturesJournal of Molecular Biology, 1967