Disposable Nafion-Coated Single-Walled Carbon Nanotube Test Strip for Electrochemical Quantitative Determination of Acetaminophen in a Finger-Prick Whole Blood Sample

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Abstract
A disposable electrochemical test strip for quantitative point-of-care (POC) determination of acetaminophen (paracetamol) in plasma and finger-prick whole blood was fabricated. The industrially scalable dry transfer process of single-walled carbon nanotubes (SWCNT) and screen-printing of silver was combined to produce integrated electrochemical test-strips. Nafion coating stabilized the potential of the Ag reference electrode and enabled the selective detection in spiked plasma as well as in whole blood samples. The test strips were able to detect acetaminophen in small 40 µL samples with a detection limit of 0.8 µM and wide linear range from 1 µM to 2 mM, well within the required clinical range. After a simple 1:1 dilution of plasma and whole blood, quantitative detection with good recoveries of 79% in plasma and 74% in whole blood were achieved. These results strongly indicate that these electrodes can be used directly to determine the unbound acetaminophen fraction without the need for any additional steps. The developed test strip shows promise as a rapid and simple POC quantitative acetaminophen assay.
Funding Information
  • Aalto-Yliopisto
  • Orionin Tutkimussäätiö
  • Tekes (211637, 2117731)

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