A simple and fast method for the isolation of basolateral plasma membranes from rat small-intestinal epithelial cells
Authors:
- V ScaleraVSV Scalera
- C StorelliCSC Storelli
- C Storelli-JossCSC Storelli-Joss
- W HaaseWHW Haase
- H MurerHMH Murer
- 15 January 1980
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 186 (1) , 177-181
Abstract
A method was developed for the analytical and preparative isolation of basolateral plasma membranes from rat small intestine. They were separated on a self-orientating Percoll (modified colloidal silica) gradient starting with a heavy microsomal-membrane fraction and involving centrifugation at 48,000 g for 1 h. (Na+ + K+)-stimulated ATPase activity, used as a marker enzyme for the basolateral plasma membrane, is enriched 20-fold compared with that found in the homogenate of isolated intestinal epithelial cells.Keywords
This publication has 11 references indexed in Scilit:
- Analytical isolation of plasma membranes of intestinal epithelial cells: Identification of Na, K-ATPase rich membranes and the distribution of enzyme activitiesThe Journal of Membrane Biology, 1976
- The surface membrane of the small intestinal epithelial cell. I. Localization of adenyl cyclaseBiochimica et Biophysica Acta (BBA) - Biomembranes, 1976
- Transport of p-aminohippuric acid by plasma membrane vesicles isolated from rat kidney cortexPflügers Archiv - European Journal of Physiology, 1976
- Basolateral plasma membranes of intestinal epithelial cells. Identification by lactoperoxidase-catalysed iodination and isolation after density perturbation with digitoninBiochemical Journal, 1975
- Glucose transport in isolated brush-border and lateral-basal plasma-membrane vesicles from intestinal epithelila cellsBiochimica et Biophysica Acta (BBA) - Biomembranes, 1974
- Preparation and characterization of the lateral and basal plasma membranes of the rat intestinal epithelial cellBiochemical Journal, 1972
- Differential isolation of microvillous and basolateral plasma membranes from intestinal mucosa: Mutually exclusive distribution of digestive enzymes and ouabain-sensitive ATPaseBiochimica et Biophysica Acta (BBA) - Biomembranes, 1972
- FURTHER BIOCHEMICAL AND MORPHOLOGICAL STUDIES OF GRANULE FRACTIONS FROM RABBIT HETEROPHIL LEUKOCYTESJournal of Cell Biology, 1970
- Distribution of (Na+-K+-stimulated ATPase activity in rat intestinal mucosaBiochimica et Biophysica Acta (BBA) - Biomembranes, 1969
- AN ELECTRON-TRANSPORT SYSTEM ASSOCIATED WITH THE OUTER MEMBRANE OF LIVER MITOCHONDRIAJournal of Cell Biology, 1967