Detection of Herpesvirus Type-Specific Antibody by a Micro Solid Phase Radioimmunometric Assay

Authors:
  • GDGordon R. Dreesman
    Gordon R. Dreesman
  • DMDavid O. Matson
    David O. Matson
  • RCRichard J. Courtney
    Richard J. Courtney
  • EAErvin Adam
    Ervin Adam
  • JMJoseph L. Melnick
    Joseph L. Melnick
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Abstract
A micro solid phase radioimmunometric assay has been developed which measures type-specific antibody in human sera to herpes simplex virus type 1 (HSV-1) and type 2 (HSV-2). The test is performed by coating wells of microtiter plates with purified HSV-specified glycoproteins, VP123 and VP119, derived from HSV-1- and HSV-2-infected cells, respectively. The coated wells are then incubated with diluted unadsorbed sera and subsequently with radio-iodinated goat anti-human IgG reagent. Because these major viral envelope glycoproteins contain type-specific antigenic determinants, type-specific antibodies are preferentially detected in our assay.