Platelet activating factor (PAF‐acether) promotes an early degradation of phosphatidylinositol‐4,5‐biphosphate in rabbit platelets

Authors:
  • GMGérard Mauco
    Gérard Mauco
  • CHChap Hugues
    Chap Hugues
  • DLDouste-Blazy Louis
    Douste-Blazy Louis
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Abstract
32P‐Labelled washed rabbit platelets were incubated with 0.6 nM platelet activating factor (PAF‐acether), giving a full aggregation and release response within 30–60 s. The major phospholipid changes observed under these conditions were: (1) An increased labelling of phosphatidic acid (PA) within 10 s and of phosphatidylinositol (MPI) at 30 s, reflecting the activation of the MPI cycle via the cytosolic phospholipase C; (2) an enhancement of phosphatidylinositol‐4‐phosphate (DPI) and phosphatidylinositol‐4,5‐bisphosphate (TPI) labelling at later incubation times; (3) an early degradation of TPI with a counterbalancing formation of DPI. The latter changes suggest a receptor‐mediated stimulation of TPI‐phosphomonoesterase, the role of which in the mechanism of platelet activation is discussed.

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