Molecular cloning of human prostate specific antigen cDNA
- 20 April 1987
- journal article
- Published by Wiley in FEBS Letters
- Vol. 214 (2) , 317-322
Abstract
A λgt11 clone encoding prostate specific antigen has been isolated from a human prostate cDNA library. The cDNA insert of 1415 nucleotides hybridizes specifically to a prostate mRNA species of 1.5 kb. The nucleotide sequence codes for part of a signal peptide, a short propiece and the mature protein of 237 amino acid residues. The M r for the non‐glycosylated protein was 26 089. One potential site for N‐linked carbohydrate attachment was identified. The primary structure shows extensive homology with proteases of the kallikrein family.Keywords
This publication has 23 references indexed in Scilit:
- Nucleotide sequence of cloned cDNA for human pancreatic kallikreinBiochemistry, 1985
- A kallikrein-like serine protease in prostatic fluid cleaves the predominant seminal vesicle protein.Journal of Clinical Investigation, 1985
- Tissue-specific expression of kallikrein-related genes in the ratBiochemistry, 1985
- Amino acid sequence of the predominant basic protein in human seminal plasmaFEBS Letters, 1985
- Yeast RNA Polymerase II Genes: Isolation with Antibody ProbesScience, 1983
- Structure of mouse kallikrein gene family suggests a role in specific processing of biologically active peptidesNature, 1983
- Refined 2 Å X-ray crystal structure of porcine pancreatic kallikrein A, a specific trypsin-like serine proteinaseJournal of Molecular Biology, 1983
- TECHNOLOGICAL EXAMINATION OF LOW‐FIRED TERRACOTTA STATUES FROM AYIA IRINI, KEAArchaeometry, 1982
- Isolation of biologically active ribonucleic acid from sources enriched in ribonucleaseBiochemistry, 1979
- RNA molecular weight determinations by gel electrophoresis under denaturing conditions, a critical reexaminationBiochemistry, 1977