SEPARATION OF CLOSTRIDIUM BOTULINUM TYPE A DERIVATIVE TOXIN INTO TWO FRAGMENTS
Authors:
- Shunji KozakiSKShunji Kozaki
- Shiro TogashiSTShiro Togashi
- Genji SakaguchiGSGenji Sakaguchi
- 1 January 1981
- journal article
- research article
- Published by Editorial Committee of Japanese Journal of Infectious Diseases, National Institute of Infectious Dis in Japanese Journal of Medical Science and Biology
- Vol. 34 (2) , 61-68
Abstract
Two fragments with molecular weights of 105,000 (fragment I) and 58,000 (fragment II) were separated chromatographically from each other after Clostridium botulinum type A derivative toxin adsorbed onto a QAE-Sephadex column was treated with dithiothreitol and urea. They were antigenic and formed crossing precipitin lines against anti-derivative toxin in agar gel diffusion tests. Upon removal of dithiothreitol and urea by dialysis, the two fragments reassembled to reconstruct the derivative toxin molecule.Keywords
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