Figure 2 from Novel Mechanism of and Therapeutic Approach for Anthracycline-Induced Cardiotoxicity

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Abstract
Doxorubicin induces TOP2B overexpression in the AIC mouse model. A, Western blot analysis showing increased expression of TOP2B protein in the AIC mouse model treated with doxorubicin (5 mg/kg/week, i.v., for four consecutive weeks) in mice with AIC. B, IHC staining confirming TOP2B overexpression in tissue samples from the same model. C, Immunofluorescence staining of cardiac sections demonstrating increased nuclear TOP2B (red) in cardiomyocytes following doxorubicin treatment. cTnI (green) marks cardiomyocytes, and DAPI (blue) labels nuclei. Merged images show enhanced nuclear localization of TOP2B in cardiomyocytes of doxorubicin-treated hearts relative to controls. Scale bars as indicated.