HPLC determination of the total content of aflatoxins in naturally contaminated eggs in free and conjugate forms
Authors:
- 1 October 1987
- journal article
- research article
- Published by Taylor & Francis in Food Additives & Contaminants: Part A
- Vol. 4 (4) , 407-414
Abstract
A study was undertaken to evaluate the total aflatoxin content in naturally contaminated eggs. Two pools of eggs from laying hens were collected after 2 and 7 days of treatment with aflatoxin B1 (AFB1). An HPLC method has been developed for the determination of AFB1, aflatoxin M1 (AFM1), aflatoxin B2a (AFB2a) and aflatoxicol (Ro) both in free form and after release from water‐soluble conjugates. The bound form was cleaned up after acid hydrolysis of the aqueous phase. Eggs collected after 2 days of treatment revealed residues of AFB1, AFB2a and Ro in the organic phase, but none in the aqueous portion. After 7 days of treatment both AFB1 and its hydroxy derivative were found in the organic phase but the aqueous portion showed only hydroxylated metabolites accounting for 35% of the total aflatoxin content.Keywords
This publication has 10 references indexed in Scilit:
- Aflatoxin deposition and clearance in the eggs of laying hensFood and Chemical Toxicology, 1985
- Characterization of water-soluble glucuronide and sulphate conjugates of aflatoxin B1. 1. Urinary excretion in monkey, rat and mouseFood and Chemical Toxicology, 1985
- Urinary excretion of thiol conjugates of aflatoxin B1 in rats and hamstersCancer Letters, 1984
- Aflatoxicol and Aflatoxins B1 and M1 in Eggs and Tissues of Laying Hens Consuming Aflatoxin-Contaminated FeedPoultry Science, 1983
- Metabolite distribution and rate of residue clearance in Turkeys fed a diet containing aflatoxin B1Food and Chemical Toxicology, 1983
- The formation of 2,3-dihydroxy-2,3-dihydro-aflatoxin B1 by the metabolism of aflatoxin B1 by liver microsomes isolated from certain avian and mammalian species and the possible role of this metabolite in the acute toxicity of aflatoxin B1Toxicology and Applied Pharmacology, 1981
- The role of aflatoxin metabolism in its toxic lesionToxicology and Applied Pharmacology, 1976
- Metabolism of Foreign Compounds by Gastrointestinal MicroorganismsPharmacological Reviews, 1973
- Metabolism as a factor in determining the toxic action of the aflatoxins in different animal speciesFood and Cosmetics Toxicology, 1973
- The in vitro reduction of aflatoxins B1 and B2 by soluble avian liver enzymesFood and Cosmetics Toxicology, 1971