Dendritic Oligoglycerol Regioisomer Mixtures and Their Utility for Membrane Protein Research
Open Access
- 7 October 2020
- journal article
- research article
- Published by Wiley in Chemistry – A European Journal
- Vol. 27 (7) , 2537-2542
Abstract
Dendrons are an important class of macromolecules that can be used for a broad range of applications. Recent studies have indicated that mixtures of oligoglycerol detergent (OGD) regioisomers are superior to individual regioisomers for protein extraction. The origin of this phenomenon remains puzzling. Here we discuss the synthesis and characterization of dendritic oligoglycerol regioisomer mixtures and their implementation into detergents. We provide experimental benchmarks to support quality control after synthesis and investigate the unusual utility of OGD regioisomer mixtures for extracting large protein quantities from biological membranes. We anticipate that our findings will enable the development of mixed detergent platforms in the future.Keywords
Funding Information
- H2020 European Research Council (695511)
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