Functional characterization of a delta 6-desaturase gene from the black seabream (Acanthopagrus schlegeli)
- 12 February 2011
- journal article
- Published by Springer Nature in Biotechnology Letters
- Vol. 33 (6) , 1185-1193
Abstract
Delta 6-fatty acid desaturase (D6DES) is used in the synthesis of polyunsaturated fatty acids (PUFAs) from microorganisms to higher animals, including arachidonic acid (ARA) and eicosapentaenoic acid (EPA). A 1,338 bp full-length cDNA encoding D6DES was cloned from Acanthopagrus schlegeli (AsD6DES) through degenerate- and RACE-PCR methods. A recombinant vector expressing AsD6DES (pYES-AsD6DES) was subsequently constructed and transformed into Saccharomyces cerevisiae to test the enzymatic activity of AsD6DES towards the production of n-6 and n-3 fatty acids. The exogenously expressed AsD6DES produced γ-linolenic acid (18:3 n-6) and stearidonic acid (18:4n-3) at 26 and 36% from exogenous linoleic acid (18:2n-6) and α-linolenic acid (18:3n-3), respectively, indicating that it is essentially a delta 6-fatty acid desaturase.Keywords
This publication has 18 references indexed in Scilit:
- Multiple genes for functional ∆6 fatty acyl desaturases (Fad) in Atlantic salmon (Salmo salar L.): Gene and cDNA characterization, functional expression, tissue distribution and nutritional regulationBiochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids, 2010
- Docosahexaenoic acid (DHA), an essential fatty acid for the proper functioning of neuronal cells: their role in mood disordersGrasas y Aceites, 2009
- Enhancing Plant Seed Oils for Human NutritionPlant Physiology, 2008
- Relief for fish stocks: oceanic fatty acids in transgenic oilseedsTrends in Plant Science, 2005
- Cloning and nutritional regulation of a Δ6-desaturase-like enzyme in the marine teleost gilthead seabream (Sparus aurata)Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology, 2003
- High-Level Production of γ-Linolenic Acid in Brassica juncea Using a Δ6 Desaturase from Pythium irregularePlant Physiology, 2002
- Polyunsaturated fatty acid contents of some traditional fish and shrimp paste condiments of the PhilippinesFood Chemistry, 2001
- Cloning, tissue distribution and nutritional regulation of a Δ6-desaturase-like enzyme in rainbow troutComparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology, 2001
- Identification of a Δ4 Fatty Acid Desaturase fromThraustochytrium sp. Involved in the Biosynthesis of Docosahexanoic Acid by Heterologous Expression inSaccharomyces cerevisiae and Brassica junceaJournal of Biological Chemistry, 2001
- In vivo metabolism of [1-14C]linolenic acid (18:3(n − 3)) and [1-14C]eicosapentaenoic acid (20:5(n − 3)) in a marine fish: Time-course of the desaturation/elongation pathwayBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1994