Highlylanddenantioselective variants of horseradish peroxidase discovered by an ultrahigh-throughput selection method
- 18 November 2008
- journal article
- Published by National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 105 (46) , 17694-17699
Abstract
A highly efficient selection method for enhanced enzyme enantioselectivity based on yeast surface display and fluorescence-activated cell sorting (FACS) is developed and validated. Its application to horseradish peroxidase has resulted in enzyme variants up to 2 orders of magnitude selective toward either substrate enantiomer at will. These marked improvements in enantioselectivity are demonstrated for the surface-bound and soluble enzymes and rationalized by computational docking studies.Keywords
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