DIFFERENT LETHAL EFFECTS OF MITOMYCIN C AND ACTINOMYCIN D DURING THE DIVISION CYCLE OF HELA CELLS
Authors:
Open Access
- 1 September 1968
- journal article
- research article
- Published by Rockefeller University Press in Journal of Cell Biology
- Vol. 38 (3) , 477-482
Abstract
The lethal actions of mitomycin C and actinomycin D were followed during the division cycle of HeLa cells. The cells were most susceptible to a 2 hr pulse of mitomycin C during the G1 phase, whereas their sensitivity to actinomycin D was most pronounced in the S phase. Posttreatment of the cells with acetoxycycloheximide, a potent inhibitor of protein synthesis, increased the survival (colony-forming ability) of cells treated with mitomycin C but had very little effect on the survival of cells treated with actinomycin D. The significance of these findings is discussed.Keywords
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