A simple method for isolating viable mature parasites of Plasmodium falciparum from cultures
Authors:
- A.R. DluzewskiADA.R. Dluzewski
- Irene T. LingILIrene T. Ling
- Kaveri RangachariKRKaveri Rangachari
- P.A. BatesPBP.A. Bates
- R.J.M. WilsonRWR.J.M. Wilson
- 1 January 1984
- journal article
- Published by Oxford University Press (OUP) in Transactions of the Royal Society of Tropical Medicine and Hygiene
- Vol. 78 (5) , 622-624
Abstract
Mature asexual parasites from cultures of knobby or knobless clones of P. falciparum containing 5 to 10% late forms were harvested by layering up to 10(9) erythrocytes on 3 ml Percoll. The density of the Percoll was adjusted to between 1.081 and 1.091 g cm-3, depending on the maturity of the parasites. Centrifugation at 1500 g for 10 min produced a sharp band at the interface containing mature parasites with a purity averaging 86%. Washed parasites derived from the layer or pellet showed good viability in vitro.Keywords
This publication has 9 references indexed in Scilit:
- Biosynthesis and processing of a Plasmodium falciparum schizont antigen recognized by immune serum and a monoclonal antibody.Journal of Experimental Medicine, 1982
- Concentration of Plasmodium falciparum-Infected Erythrocytes by Density Gradient Centrifugation in PercollJournal of Parasitology, 1982
- Plasmodium falciparum: Assay of invasion of erythrocytesExperimental Parasitology, 1981
- S-antigens and isozymes in strains of Plasmodium falciparumTransactions of the Royal Society of Tropical Medicine and Hygiene, 1981
- Plasmodium falciparum: Loss of knobs on the infected erythrocyte surface after long-term cultivationExperimental Parasitology, 1979
- Synchronization of Plasmodium falciparum Erythrocytic Stages in CultureJournal of Parasitology, 1979
- Concentration from Continuous Culture of Erythrocytes Infected with Trophozoites and Schizonts of Plasmodium FalciparumThe American Journal of Tropical Medicine and Hygiene, 1978
- Separation of viable schizont-infected red cells ofPlasmodium falciparumfrom human bloodPathogens and Global Health, 1978
- Human Malaria Parasites in Continuous CultureScience, 1976