Purification by silica gel chromatography using dialysis tubing and characterization of sophorolipids produced from Candida bombicola grown on glucose and arachidonic acid
Authors:
- 8 November 2006
- journal article
- Published by Springer Nature in Biotechnology Letters
- Vol. 29 (2) , 267-272
Abstract
The yeast Candida bombicola (ATCC 22214) grown on primary carbon source glucose (100 g l−1) and secondary carbon, arachidonic acid (2 g l−1) produced mixture of sophorolipids up to 1.44 g l−1. The crude product was a heterogeneous mixture of sophorolipids, which are glycolipids of sophorose linked to the fatty acid through glycosidic bond between ω and ω−1 carbon of arachidonic acid. The derived sophorolipids were isolated by silica gel chromatography using dialysis tubing. The purified sophorolipids were characterized by ESI-MS and FT-IR. Acid hydrolysis of the resolved sophorolipids were characterized by ESI-MS for the presence of 20-hydroxy-5Z,8Z,11Z,14Z-eicosatetraenoic acid (20-HETE) and 19-hydroxy-5Z,8Z,11Z,14Z-eicosatetraenoic acid (19-HETE), compounds of pronounced pharmaceutical importance.Keywords
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