DNA Barcoding and Allele-Specific PCR Discrimination of Glasswort Ecotypes from Apulia Region (Southern Italy)

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Abstract
In the scenario of ongoing climate changes, the selection of plant genotypes with high salt tolerance is emerging as the most sustainable strategy to safeguard crop yield and quality and make productive use of salinized soils. Glassworts are annual and perennial halophytes found in inner and coastal wastelands, indistinctly consumed as high-nutritional green vegetables. Traditional taxonomic classification based on morphological traits can be very challenging in glasswort, due to phenotypic plasticity, reduced plant morphology, and inbreeding. In this work, we used DNA-based molecular tools to overcome such constraints and assess inter-generic and inter-specific genetic diversity in a collection of ecotypes from different Apulian areas. A fast and reliable Allele-Specific PCR assay was optimized to enable molecular detection of annual and perennial genera. Species-level classification was obtained through a similarity- and phylogeny-based approach relying on matK and rbcL DNA barcoding. Combined DNA tools identified perennial samples as Sarcocornia fruticosa and Arthrocaulon macrostachyum, along with annual Salicornia europaea, and phylogenetic trees unveiled genetic distances between glassworts, which clustered according to life cycle. The relationship between genotypes and nutritional profiles was finally investigated, suggesting that environmental factors may play a predominant role over taxonomic relatedness in shaping interspecific differences in nutrient composition of the analyzed samples.
Funding Information
  • European Commission (D.D. 1032 17/06/2022, CN00000022)

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