Tissue-Engineered Skeletal Muscle Organoids for Reversible Gene Therapy
Authors:
- Michael Del TattoMTMichael Del Tatto
- Janet ShanskyJSJanet Shansky
- Julie LemaireJLJulie Lemaire
- Albert ChangACAlbert Chang
- Francis PayumoFPFrancis Payumo
- Peter LeePLPeter Lee
- Amy GoodyearAGAmy Goodyear
- Latasha RavenLRLatasha Raven
- 10 November 1996
- journal article
- Published by SAGE Publications in Human Gene Therapy
- Vol. 7 (17) , 2195-2200
Abstract
Genetically modified murine skeletal myoblasts were tissue engineered in vitro into organ-like structures (organoids) containing only postmitotic myofibers secreting pharmacological levels of recombinant human growth hormone (rhGH). Subcutaneous organoid implantation under tension led to the rapid and stable appearance of physiological sera levels of rhGH for up to 12 weeks, whereas surgical removal led to its rapid disappearance. Reversible delivery of bioactive compounds from postmitotic cells in tissue engineered organs has several advantages over other forms of muscle gene therapy.Keywords
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