Biotransformation of puerarin into puerarin-6″-O-phosphate by Bacillus cereus
Authors:
- 1 February 2012
- journal article
- Published by Oxford University Press (OUP) in Journal of Industrial Microbiology and Biotechnology
- Vol. 39 (2) , 299-305
Abstract
The biotransformation of puerarin catalyzed by Bacillus cereus NT02 was studied. A primary screening was carried out using 307 strains of bacteria isolated from soil which were able to grow in the presence of puerarin. Strain NT02, identified as B. cereus, was able to convert puerarin into puerarin-6″-O-phosphate. Under the optimum conditions, resting cells of B. cereus NT02 converted 27% of added 0.4 g/l puerarin into puerarin-6″-O-phosphate that was characterized by MS, 13C NMR, 31P NMR. The activity of puerarin-6″-O-phosphate was 25 times higher than that of puerarin in the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical-scavenging system. The water solubility of puerarin-6″-O-phosphate was 85.4 times higher than that of puerarin.Keywords
This publication has 21 references indexed in Scilit:
- Antimicrobial properties of prunin, a citric flavanone glucoside, and its prunin 6″-O-lauroyl esterJournal of Applied Microbiology, 2010
- Isolation and Identification of Chlorinated Genistein from Actinoplanes sp. HBDN08 with Antioxidant and Antitumor ActivitiesJournal of Agricultural and Food Chemistry, 2009
- Quantitative determination of puerarin in dog plasma by HPLC and study on the relative bioavailability of sustained release tabletsJournal of Pharmaceutical and Biomedical Analysis, 2006
- Chrysin and its phosphate ester inhibit cell proliferation and induce apoptosis in Hela cellsBioorganic & Medicinal Chemistry, 2004
- Effects of Purified Puerarin on Voluntary Alcohol Intake and Alcohol Withdrawal Symptoms in P Rats Receiving Free Access to Water and AlcoholJournal of Medicinal Food, 2004
- An Improved Method for Lewis Acid Catalyzed Phosphoryl Transfer with Ti(t-BuO)4The Journal of Organic Chemistry, 2003
- Evaluation of the Estrogenic Effects of Legume Extracts Containing PhytoestrogensJournal of Agricultural and Food Chemistry, 2003
- HPLC Method for Evaluation of the Free Radical-scavenging Activity of Foods by Using 1,1-Diphenyl-2-picrylhydrazylBioscience, Biotechnology, and Biochemistry, 1998
- Synthesis of inositol 2-phosphate-quercetin conjugatesCarbohydrate Research, 1996
- Antioxidative sulphated flavonoids in leaves of Polygonum hydropiperPhytochemistry, 1994