Determination of orotic acid (vitamin B 13 ) in human serum and urine by differential-pulse polarography

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Abstract
A differential-pulse polarographic method for the determination of orotic acid in urine and human serum is proposed. The effects of the amount of sample taken and the concentration of HClO4 present were investigated. The detection limit was 1.54 µg ml–1. The standard deviation of the determination of 15.4 µg ml–1 of orotic acid in 5 ml of urine was 0.50 µg ml–1 and that of the determination of 15.4 µg of orotic acid in 1 ml of human serum was 0.28 µg ml–1.