Reactive Oxygen Species and X-Ray Disrupted Spontaneous [Ca2+]iOscillation in Alveolar Macrophages

Authors:
  • HYHong Ye
    Hong Ye
  • DMDa-Quan Meng
    Da-Quan Meng
  • PCPeng-Cheng Cai
    Peng-Cheng Cai
  • LZLi-Ping Zhu
    Li-Ping Zhu
  • QTQiu Tang
    Qiu Tang
  • ZLZhi-Xiong Long
    Zhi-Xiong Long
  • QZQiong Zhou
    Qiong Zhou
  • YJYang Jin
    Yang Jin
  • JXJian-Bao Xin
    Jian-Bao Xin
  • XTXiao-Nan Tao
    Xiao-Nan Tao
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Abstract
Radiation leads to a rapid burst of reactive oxygen species (ROS), which is considered to be one of the major causes of radiation-induced injury. ROS have previously been shown to induce changes in cytosolic Ca2+ ([Ca2+]i) including [Ca2+]i oscillation. However, the role of radiation in [Ca2+]i oscillation is poorly understood. The purpose of this study was to identify the effect of ROS and X ray on [Ca2+]i oscillation, as well as their role in radiation-induced lung injury. Alveolar macrophages were cultured in the absence and presence of different doses of hydrogen peroxide (H2O2) or exposed to X-ray irradiation with or without pretreatment of diphenyleneiodonium chloride (DPI, an inhibitor of NADPH oxidases) or tetrandrine (TET, a calcium entry blocker) and cytosolic Ca2+ concentration was detected by fluorescent Ca2+ indicator Fura-2. Rat radiation lung injury was induced in vivo by using 40 Gy X ray and DPI or TET was used to prevent radiation-induced lung injury. The results showed that there was sp...

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