Optimisation of the in‐vivo assay conditions for nitrate reductase in barley (Hordeum vulgare L. cv. Igri)
Author:
- 1 July 1984
- journal article
- research article
- Published by Wiley in Journal of the Science of Food and Agriculture
- Vol. 35 (7) , 725-730
Abstract
Assay conditions in vivo for determining nitrate reductase activity (NRA) in shoot and root tissues of winter barley (Hordeum vulgare L. cv. Igri) were studied. The composition, pH and temperature of the incubation medium, and the size of leaf slice were varied. Maximum leaf NRA was obtained with 5 mm transverse slices incubated at 32oC in a medium containing 0.1M phosphate buffer, pH 7.0, 0.02M KNO3, 30 ml litre−1 propan‐1‐ol and 0.1 ml litre−1 Triton X‐100. The optimum assay conditions for roots were similar to those for leaves, except that the pH was 7.5. There was negligible nitrite reductase activity in leaves incubated in the dark.Keywords
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