Cloning of bovine growth hormone gene and its expression in bacteria
Authors:
- E. KeshetEKE. Keshet
- A. RosnerARA. Rosner
- Y. BernsteinYBY. Bernstein
- H. AvivHAH. Aviv
Open Access
- 10 January 1981
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 9 (1) , 19-30
Abstract
A hybrid plasmid was constructed containing β-lactamase gene of plasmid pBR322 and cloned coding sequences of bovine growth hormone (BGH). The constructed plasmid contains all DNA sequences required to encode BGH, and when used as a hybridization probe it detects one growth hormone gene in the bovine genome. The cloned DNA sequences are inserted into the β-lactamase gene in the correct reading frame for BGH synthesis. The hybrid gene is expressed in bacteria and the product, a fused β-lactamase-bovine growth hormone protein, is specifically immunoprecipitated with anti-serum to BGH Unlike β-lactamase, very little growth hormone containing sequences can be detected in the periplasmic space.Keywords
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