SEPARATION OF STEROLS AND TRITERPENE ALCOHOLS FROM UNSAPONIFIABLE FRACTIONS OF THREE PLANT SEED OILS

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Abstract
Preparative HPLC was used to separate sterols and triterpene alcohols from the unsaponifiable matters in plant oils from Camellia weiningensis L., Brassica juncea L. and Microula sikkimensis. The isolated sterols and triterpene alcohols were acetylated and further purified by AgNO3 impregnated silica gel preparative thin layer chromatography (TLC). The isolated acetyl derivatives of sterols and triterpene alcohols were identified by melting point, specific rotation, infrared and mass spectrometry. The sterols were brassicasterol, campesterol, stigmasterol, β‐sitosterol and Θ5‐avenasterol, Θ7‐avenasterol, Θ7‐stigmastenol and α‐spinasterol. The triterpene alcohols were cycloartanol, cycloartenol, 24‐methylenecycloartanol, cyclobranol, dammaradienol, tirucalla‐7,24‐dienol, butyrospermol, β‐amyrin, germanicol, α‐4‐taraxasterol and lupeol.