Production of Native-Type Streptoverticillium mobaraense Transglutaminase in Corynebacterium glutamicum
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Open Access
- 1 May 2003
- journal article
- research article
- Published by American Society for Microbiology in Applied and Environmental Microbiology
- Vol. 69 (5) , 3011-3014
Abstract
We previously observed secretion of active-form transglutaminase in Corynebacterium glutamicum by coexpressing the subtilisin-like protease SAM-P45 from Streptomyces albogriseolus to process the prodomain. However, the N-terminal amino acid sequence of the transglutaminase differed from that of the native Streptoverticillium mobaraense enzyme. In the present work we have used site-directed mutagenesis to generate an optimal SAM-P45 cleavage site in the C-terminal region of the prodomain. As a result, native-type transglutaminase was secreted.Keywords
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