Total synthesis of a human leukocyte interferon gene
Authors:
- Michael D. EdgeMEMichael D. Edge
- April R. GreeneAGApril R. Greene
- Gillian R. HeathcliffeGHGillian R. Heathcliffe
- Peter A. MeacockPMPeter A. Meacock
- Wolfgang SchuchWSWolfgang Schuch
- Denis B. ScanlonDSDenis B. Scanlon
- Thomas C. AtkinsonTAThomas C. Atkinson
- Clive R. NewtonCNClive R. Newton
- Alexander F. MarkhamAMAlexander F. Markham
- 1 August 1981
- journal article
- research article
- Published by Springer Nature in Nature
- Vol. 292 (5825) , 756-762
Abstract
A 514-base pair fragment of double-stranded DNA coding for human interferon-alpha 1 (166 amino acid residues), and containing initiation and termination signals plus appropriate restriction enzyme sites for plasmid insertion, has been totally synthesized. The synthesis involved preparation of 66 oligodeoxyribonucleotides, ranging in size from 14 to 21 residues, plus 1 deoxydecanucleotide, by rapid, solid phase procedures, and enzymatic ligation of the oligonucleotides. After ligation of the synthetic gene to a plasmid vector and transformation of Escherichia coli, clones containing the anticipated gene sequence were obtained.Keywords
This publication has 50 references indexed in Scilit:
- Two interferon mRNAs in human fibroblasts: in vitro translation and Escherichia coli cloning studies.Proceedings of the National Academy of Sciences, 1980
- At Least Three Human Type α Interferons: Structure of α2Science, 1980
- Human leukocyte and fibroblast interferons are structurally relatedNature, 1980
- The nucleotide sequence of human fibroblast interferon cDNAGene, 1980
- The nucleotide sequence of a cloned human leukocyte Interferon cDNAGene, 1980
- Isolation and structure of a human fibroblast interferon geneNature, 1980
- The amino-terminal sequence of human fibroblast interferon as deduced from reverse transcripts obtained using synthetic oligonucleotide primersNucleic Acids Research, 1980
- Interferons and the immune systemNature, 1980
- Construction and identification of a bacterial plasmid containing the human fibroblast interferon gene sequence.Proceedings of the Japan Academy, Series B, 1979
- The Interferon System1979