Separation of Monovinyl and Divinyl Protochlorophyllides and Chlorophyllides from Etiolated and Phototransformed Cucumber Cotyledons

Authors:
  • PCPaul A. Castelfranco
    Paul A. Castelfranco
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Abstract
A method was developed to separate the monovinyl and divinyl forms of protochlorophyllide and chlorophyllide by high pressure liquid chromatography using a silicic acid column coated with dodecyl residues and a moving phase containing the lipophilic cation, tetrabutyl ammonium. The solvent was 70% methyl alcohol containing varying amounts of methyl ethyl ketone. The separation was carried out at 0 degrees C. This method was used to test and confirm a previous report that, in cucumber cotyledons, divinyl protochlorophyllide is phototransformed to give divinyl chlorophyllide, which is biologically unstable and disappears rapidly in the dark.

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