Automation Protocol for Plasmid DNA Extraction from E. coli v1

Authors:
  • NANina Alperovich
    Nina Alperovich
  • JRJane Romantseva
    Jane Romantseva
  • NVNot Provided Olga Vasilyeva
    Not Provided Olga Vasilyeva
  • DRDavid Ross
    David Ross
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Abstract
Here, we describe a protocol for automated extraction of plasmid DNA from 24 E. coli cultures. The protocol uses an automated liquid handler and a positive pressure filter press. It is based on the QIAprep Spin Miniprep Kit (Qiagen, 27106). This protocol requires an automated liquid handler (Hamilton Robotics, STAR) with both 8-channel and 96-channel pipetting heads and positive pressure filter press (Hamilton, MPE2). It also requires a centrifuge with a 96-well plate rotor capable of at least 3878 g.