Strategies for Creating Structure-Switching Aptamers

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Abstract
Aptamer biosensor that can switch its structure upon target binding offers a powerful strategy for molecular detection. However, the process of converting an aptamer into a “structure-switching” biosensor is challenging and often relies on trial-and-error without established design principles. In this Sensor Issues, we examine a variety of design approaches for incorporating structure-switching functionality into existing aptamers, and provide thermodynamic analyses to highlight the variables that most strongly influence their performance. Finally, we also describe emerging efforts for incorporating the structure-switching functionality directly into the aptamer selection process.
Funding Information
  • NIH Office of the Director (OT2 OD025342)
  • Bill and Melinda Gates Foundation
  • National Institute of General Medical Sciences (R01 GM129313-01)

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