Plasmid Detection and Sizing in Single Colony Lysates
Author:
- 28 January 1977
- journal article
- research article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 195 (4276) , 393-394
Abstract
A simple and contained procedure for the rapid assay of the presence and size of plasmids similar to Col E1 is described. Bacteria are picked from an agar plate with a toothpick, lysed with dodecyl sulfate and heat, and placed directly on an agarose gel for electrophoresis.Keywords
This publication has 8 references indexed in Scilit:
- Novel Screening Procedure for Recombinant PlasmidsScience, 1977
- Characterization of a mini-ColC1 plasmidJournal of Bacteriology, 1976
- Indirect selection of bacterial plasmids lacking identifiable phenotypic propertiesJournal of Bacteriology, 1975
- Hybridization of labeled RNA to DNA in agarose gelsNucleic Acids Research, 1975
- Construction of φ80 dhisCarryingSalmonella typhimuriumHistidine Operon MutationsJournal of Bacteriology, 1974
- Nature of Col E 1 Plasmid Replication in Escherichia coli in the Presence of ChloramphenicolJournal of Bacteriology, 1972
- Circular DNAAnnual Review of Biochemistry, 1971
- Properties of a supercoiled deoxyribonucleic acid-protein relaxation complex and strand specificity of the relaxation eventBiochemistry, 1970