Determination of Yohimbine in Serum by High Performance Liquid Chromatography

Authors:
  • YIYoko Isii
    Yoko Isii
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Abstract
An automated method for determination of yohimbine (Yoh) in the serum was developed by means of column switching high performance liquid chromatography (HPLC). TSK-precolumn BSA-ODS and TSK-gel ODS-120T were used as a precolumn and analytical column, respectively. The wavelengths of detection were used at 280 nm (excitation) and 360 nm (emission). A 100 .mu.l serum sample is directly injected onto the precolumn. Yoh is then eluted within 30 min with an methanol-potassium phosphate buffer mixture. The analytic recoveries (99.3-110.4%), reproducibilities (within-run, C.V. < 2.27%), and detection limit (0.80 ng/ml, S/N=3) indicate that this system is suited for determination of Yoh. The five healthy volunteers received a single oral dose 10 mg of HYoh powder. The average of the maximal serum concentration and the area under the curve (AUC) from 0 to 4 h were 10.3 .+-. 0.88 ng/ml and 19.70 .+-. 0.87 ng .cntdot. ml-1, respectively. The elimination rate constant (Kel) was 0.52 .+-. 0.03 h, and biological half-life (t1/2el) was 1.32 .+-. 0.12 h.