Carboxymethylated and Sulfated Furcellaran from Furcellaria lumbricalis and Its Immobilization on PLA Scaffolds

Authors:
Open Access
Publisher website
Google Scholar
Abstract
This study involved the creation of highly porous PLA scaffolds through the porogen/leaching method, utilizing polyethylene glycol as a porogen with a 75% mass ratio. The outcome achieved a highly interconnected porous structure with a thickness of 25 μm. To activate the scaffold’s surface and improve its hydrophilicity, radiofrequency (RF) air plasma treatment was employed. Subsequently, furcellaran subjected to sulfation or carboxymethylation was deposited onto the RF plasma treated surfaces with the intention of improving bioactivity. Surface roughness and water wettability experienced enhancement following the surface modification. The incorporation of sulfate/carboxymethyl group (DS = 0.8; 0.3, respectively) is confirmed by elemental analysis and FT-IR. Successful functionalization of PLA scaffolds was validated by SEM and XPS analysis, showing changes in topography and increases in characteristic elements (N, S, Na) for sulfated (SF) and carboxymethylated (CMF). Cytocompatibility was evaluated by using mouse embryonic fibroblast cells (NIH/3T3).
Funding Information
  • Ministry of Education, Youth, and Sports of the Czech Republic (DKRVO RP/CPS/2022/005, DKRVO RP/CPS/2022/001, L2-2616, P2-0082)
  • The Slovenian Research and Innovation Agency (DKRVO RP/CPS/2022/005, DKRVO RP/CPS/2022/001, L2-2616, P2-0082)

This publication has 68 references indexed in Scilit: