Immune activation and regulatory T cells in Mycobacterium tuberculosis infected lymph nodes
Authors:
- Karima SahmoudiKSKarima Sahmoudi
- Hassan AbbassiHAHassan Abbassi
- Nada BouklataNBNada Bouklata
- Mohamed Nouredine El AlamiMAMohamed Nouredine El Alami
- Abderrahmane SadakASAbderrahmane Sadak
- Christopher BurantCBChristopher Burant
- W. Henry BoomWBW. Henry Boom
- Rajae El AouadRARajae El Aouad
- David H. CanadayDCDavid H. Canaday
- Fouad SeghrouchniFSFouad Seghrouchni
Open Access
- 8 November 2018
- journal article
- research article
- Published by Springer Nature in BMC Immunology
- Vol. 19 (1) , 1-7
Abstract
Lymph node tuberculosis (LNTB) is the most frequent extrapulmonary form of tuberculosis (TB). Studies of human tuberculosis at sites of disease are limited. LNTB provides a unique opportunity to compare local in situ and peripheral blood immune response in active Mycobacterium tuberculosis (Mtb) disease. The present study analysed T regulatory cells (Treg) frequency and activation along with CD4+ T cell function in lymph nodes from LNTB patients. Lymph node mononuclear cells (LNMC) were compared to autologous peripheral blood mononuclear cells (PBMC). LNMC were enriched for CD4+ T cells with a late differentiated effector memory phenotype. No differences were noted in the frequency and mutifunctional profile of memory CD4+ T cells specific for Mtb. The proportion of activated CD4+ and Tregs in LNMC was increased compared to PBMC. The correlation between Tregs and activated CD4+ T cells was stronger in LNMC than PBMC. Tregs in LNMC showed a strong positive correlation with Th1 cytokine production (IL2, IFNγ and TNFα) as well as MIP-1α after Mtb antigen stimulation. A subset of Tregs in LNMC co-expressed HLA-DR and CD38, markers of activation. Further research will determine the functional relationship between Treg and activated CD4+ T cells at lymph node sites of Mtb infection.Keywords
Funding Information
- Hassan IIed Academy for Sciences and techniques of Morocco (IMMGEN)
- National Institutes of Health (AI108972, P30 AI036219)
- National Institutes of Health (AI080313)
- National Institute of Allergy and Infectious Diseases (HHSN266200700022C / NO1-AI-70022)
- VA GRECC
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