Establishment and Characterization of a Buffalo (Bubalus bubalis) Mammary Epithelial Cell Line
Open Access
- 9 July 2012
- journal article
- research article
- Published by Public Library of Science (PLoS) in PLOS ONE
- Vol. 7 (7) , e40469
Abstract
The objective of this study was to establish the buffalo mammary epithelial cell line (BuMEC) and characterize its mammary specific functions. Buffalo mammary tissue collected from the slaughter house was processed enzymatically to obtain a heterogenous population of cells containing both epithelial and fibroblasts cells. Epithelial cells were purified by selective trypsinization and were grown in a plastic substratum. The purified mammary epithelial cells (MECs) after several passages were characterized for mammary specific functions by immunocytochemistry, RT-PCR and western blot. The established buffalo mammary epithelial cell line (BuMEC) exhibited epithelial cell characteristics by immunostaining positively with cytokeratin 18 and negatively with vimentin. The BuMEC maintained the characteristics of its functional differentiation by expression of β-casein, κ-casein, butyrophilin and lactoferrin. BuMEC had normal growth properties and maintained diploid chromosome number (2n = 50) before and after cryopreservation. A spontaneously immortalized buffalo mammary epithelial cell line was established after 20 passages and was continuously subcultured for more than 60 passages without senescence. We have established a buffalo mammary epithelial cell line that can be used as a model system for studying mammary gland functions.Keywords
This publication has 49 references indexed in Scilit:
- Characteristics and EGFP expression of porcine mammary gland epithelial cellsResearch in Veterinary Science, 2010
- Spontaneously immortalised bovine mammary epithelial cells exhibit a distinct gene expression pattern from the breast cancer cellsBMC Cell Biology, 2010
- The influence of extracellular matrix and prolactin on global gene expression profiles of primary bovine mammary epithelial cellsin vitroAnimal Genetics, 2010
- In Vitro Culture and Characterization of a Mammary Epithelial Cell Line from Chinese Holstein Dairy CowPLOS ONE, 2009
- Events in the immortalizing process of primary human mammary epithelial cells by the catalytic subunit of human telomeraseBiochemical Journal, 2002
- Primary Culture of Porcine Mammary Epithelial Cells as a Model System for Evaluation of Milk Protein ExpressionBioscience, Biotechnology, and Biochemistry, 2001
- Standard Karyotype of the river buffalo (Bubalus bubalis L., 2n = 50)Cytogenetic and Genome Research, 1994
- Mammary Carcinoma with Peritoneal Metastasis in a CowVeterinary Pathology, 1992
- Regulation of vimentin expression in cultured human mammary epithelial cellsDifferentiation, 1990
- 3T3-L1 Adipocytes promote the growth of mammary epitheliumExperimental Cell Research, 1984