Crystal Structure Analysis and Molecular Model of Human C3a Anaphylatoxin
Authors:
- Robert HuberRHRobert Huber
- Henning ScholzeHSHenning Scholze
- Eric P. PâquesEPEric P. Pâques
- Johann DeisenhoferJDJohann Deisenhofer
- 1 January 1980
- journal article
- research article
- Published by Walter de Gruyter GmbH in Hoppe-Seyler´s Zeitschrift Für Physiologische Chemie
- Vol. 361 (2) , 1389-1400
Abstract
Human anaphylatoxin C3a, derived from complement component C3 has been crystallized and its crystal structure determined at 3.2 A resolution based on multiple isomorphous replacement. The electron density map was interpretable in terms of the known chemical sequence and molecular model constructed. The molecule has dimensions of 42 X 22 X 16 A. It resembles a drumstick. It is constructed from two helical segments Tyr15 to Met27 and Gly46 to Ser71 connected by a loop. Residues 1 to 14 are flexible. The C-terminal residues are in irregular conformation. The crystal structure analysis establishes the disulphide linkages as Cys22-Cys49, Cys23-Cys56, Cys36-Cys57.Keywords
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