Abstract
Ten Gram-positive, aerobic bacterial strains with coccoid cells occurring singly, in pairs, tetrads and small aggregates were isolated from the surface of marble and calcareous stones. The peptidoglycan containedmeso-diaminopimelic acid as the diagnostic diamino acid. The major menaquinone was MK-9(H4). The cellular fatty acid pattern consisted mainly of iso-branched chain components. According to their phylogenetic position, the organisms are members of the familyGeodermatophilaceae, clustering close toBlastococcus aggregatusDSM 4725T. Based on a combination of phenotypic, chemotaxonomic and genotypic characteristics, strain BC444Tand seven relatives group apart from strains BC412 (=DSM 44517) and BC521 (=DSM 44518), which themselves form two individual lineages. DNA–DNA reassociation studies revealed that members of the three lineages were only remotely related toB. aggregatus DSM 4725T; as the strain-rich cluster containing BC444Tshared low DNA similarity values with strains BC412 and BC521, it will be described as a novel species ofBlastococcus, for which the nameBlastococcus saxobsidenssp. nov. is proposed. The type strain is BC444T(=DSM 44509T=NRRL 24246T).

This publication has 22 references indexed in Scilit: