Nanofluidic Devices for Rapid Continuous-Flow Bioseparation

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Abstract
Compared with conventional gel-based techniques, such as gel electrophoresis, which are routinely used for bioseparation in biology and biomedical laboratories, nanofluidic devices with regular engineered sieving structures offer the potential for faster separation, better resolution, higher throughput, and more convenient sample recovery. Here, we detail the fabrication process of a two-dimensional nanofluidic filter array device and its implementation for rapid continuous-flow separation of biomolecules such as proteins.

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