Pharmacodynamics of aminoglycosides and tetracycline derivatives against Japanese encephalitis virus
Authors:
Open Access
- 1 March 2016
- journal article
- Published by Medknow in Asian Pacific Journal of Tropical Medicine
- Vol. 9 (3) , 241-246
Abstract
To explore the antiviral activity of antibiotic compounds, mainly aminoglycosides and tetracyclines against Japanese encephalitis virus (JEV) induced infection in vitro. Antiviral activity were evaluated against JEV using cytopathic effect inhibition assay, virus yield reduction assay, caspase 3 level, extracellular viral detection by antigen capture ELISA and viral RNA levels. JEV induced cytopathic effect along with reduction of viral progeny plaque formation indicated antiviral potential of the compounds suggesting that antibiotics had broad spectrum activity. Doxycycline and kanamycin administration in dose dependent manner declined viral RNA replication. The present study shows kanamycin and doxycycline can affect virion structure and alter replication causing inhibition of JEV induced pathogenesis in vitro.Keywords
Funding Information
- ICMR SRF programme
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