Clostridium sporogenes Isolates and their Relationship to C. botulinum based on Deoxyribonucleic Acid Reassociation

Authors:
  • SNShinichi Nakamura
    Shinichi Nakamura
  • IOImiko Okado
    Imiko Okado
  • SNSatoshi Nakashio
    Satoshi Nakashio
  • SNShoki Nishida
    Shoki Nishida
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Abstract
Summary Sixty-two isolates of Clostridium sporogenes from canned foods were examined for cultural properties, heat resistance and DNA-DNA homology to Clostridium botulinum type A190. Sporulation was observed in most of 21 umbonate and rhizoidal colony-forming strains (colony-type I strains), but not in most of the 41 strains with convex and circular or crenate colonies with a mat to semi-glossy surface (colony-type II strains). More than half of the latter strains showed much higher heat resistance than the rhizoidal colony-forming strains. The DNA isolated from colony-type II strains was 81% or more homologous to C. botulinum A190 DNA, forming duplexes which had thermostabilities similar to homologous duplexes of strain A190 DNA. Colony-type I strains differed from C. botulinum by 30 to 40% DNA homology and the DNA duplexes formed between these strains and strain A190 showed ΔT m(e) values of 7.0 °C when compared with the T m(e) of homologous DNA duplexes of strain A190.